Microarray:Article Title: The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer
Article Snippet: .. Protein microarray analyses DNA encoding SNORD50A and SNORD50B was synthesized by overlapping PCR of a construct with an SP6 promoter at the 5′ end, the resulting fragment was cloned into the pCR2.1TOPO vector (Life Technologies), and RNAs were produced and labeled with Cy5 dye with a ratio of RNA:Label IT Cy5 reagent of 1:3 and a reaction time of not more then 30 min at 37 °C to achieve between 0.5 and 1 Cy5 dye unit covalently attached to each RNA used. .. For RNA incubation, the ProtoArray Human Protein Microarray v5.0 (Life Technologies) was used.
Synthesized:Article Title: The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer
Article Snippet: .. Protein microarray analyses DNA encoding SNORD50A and SNORD50B was synthesized by overlapping PCR of a construct with an SP6 promoter at the 5′ end, the resulting fragment was cloned into the pCR2.1TOPO vector (Life Technologies), and RNAs were produced and labeled with Cy5 dye with a ratio of RNA:Label IT Cy5 reagent of 1:3 and a reaction time of not more then 30 min at 37 °C to achieve between 0.5 and 1 Cy5 dye unit covalently attached to each RNA used. .. For RNA incubation, the ProtoArray Human Protein Microarray v5.0 (Life Technologies) was used.
Polymerase Chain Reaction:Article Title: The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer
Article Snippet: .. Protein microarray analyses DNA encoding SNORD50A and SNORD50B was synthesized by overlapping PCR of a construct with an SP6 promoter at the 5′ end, the resulting fragment was cloned into the pCR2.1TOPO vector (Life Technologies), and RNAs were produced and labeled with Cy5 dye with a ratio of RNA:Label IT Cy5 reagent of 1:3 and a reaction time of not more then 30 min at 37 °C to achieve between 0.5 and 1 Cy5 dye unit covalently attached to each RNA used. .. For RNA incubation, the ProtoArray Human Protein Microarray v5.0 (Life Technologies) was used.
Construct:Article Title: The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer
Article Snippet: .. Protein microarray analyses DNA encoding SNORD50A and SNORD50B was synthesized by overlapping PCR of a construct with an SP6 promoter at the 5′ end, the resulting fragment was cloned into the pCR2.1TOPO vector (Life Technologies), and RNAs were produced and labeled with Cy5 dye with a ratio of RNA:Label IT Cy5 reagent of 1:3 and a reaction time of not more then 30 min at 37 °C to achieve between 0.5 and 1 Cy5 dye unit covalently attached to each RNA used. .. For RNA incubation, the ProtoArray Human Protein Microarray v5.0 (Life Technologies) was used.
Clone Assay:Article Title: The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer
Article Snippet: .. Protein microarray analyses DNA encoding SNORD50A and SNORD50B was synthesized by overlapping PCR of a construct with an SP6 promoter at the 5′ end, the resulting fragment was cloned into the pCR2.1TOPO vector (Life Technologies), and RNAs were produced and labeled with Cy5 dye with a ratio of RNA:Label IT Cy5 reagent of 1:3 and a reaction time of not more then 30 min at 37 °C to achieve between 0.5 and 1 Cy5 dye unit covalently attached to each RNA used. .. For RNA incubation, the ProtoArray Human Protein Microarray v5.0 (Life Technologies) was used.
Plasmid Preparation:Article Title: The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer
Article Snippet: .. Protein microarray analyses DNA encoding SNORD50A and SNORD50B was synthesized by overlapping PCR of a construct with an SP6 promoter at the 5′ end, the resulting fragment was cloned into the pCR2.1TOPO vector (Life Technologies), and RNAs were produced and labeled with Cy5 dye with a ratio of RNA:Label IT Cy5 reagent of 1:3 and a reaction time of not more then 30 min at 37 °C to achieve between 0.5 and 1 Cy5 dye unit covalently attached to each RNA used. .. For RNA incubation, the ProtoArray Human Protein Microarray v5.0 (Life Technologies) was used.
Produced:Article Title: The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer
Article Snippet: .. Protein microarray analyses DNA encoding SNORD50A and SNORD50B was synthesized by overlapping PCR of a construct with an SP6 promoter at the 5′ end, the resulting fragment was cloned into the pCR2.1TOPO vector (Life Technologies), and RNAs were produced and labeled with Cy5 dye with a ratio of RNA:Label IT Cy5 reagent of 1:3 and a reaction time of not more then 30 min at 37 °C to achieve between 0.5 and 1 Cy5 dye unit covalently attached to each RNA used. .. For RNA incubation, the ProtoArray Human Protein Microarray v5.0 (Life Technologies) was used.
Labeling:Article Title: The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer
Article Snippet: .. Protein microarray analyses DNA encoding SNORD50A and SNORD50B was synthesized by overlapping PCR of a construct with an SP6 promoter at the 5′ end, the resulting fragment was cloned into the pCR2.1TOPO vector (Life Technologies), and RNAs were produced and labeled with Cy5 dye with a ratio of RNA:Label IT Cy5 reagent of 1:3 and a reaction time of not more then 30 min at 37 °C to achieve between 0.5 and 1 Cy5 dye unit covalently attached to each RNA used. .. For RNA incubation, the ProtoArray Human Protein Microarray v5.0 (Life Technologies) was used.
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